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Home Antibody All anti-COASY antibodies

Anti-COASY TRUEMAB Antibody Clone 2H1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA503343
  • COASY mouse monoclonal antibody,clone 2H1
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403068) , 20ug Explanation
100ul 325 In Stock
CF503343
  • Carrier-free (BSA/glycerol-free) COASY mouse monoclonal antibody,clone 2H1
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IHC(2)
FC(1)
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Also for COASY (NM_025233)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human COASY(NP_079509) produced in HEK293T cell.
Clone NameClone 2H1 IsotypeIgG1
Species ReactivityHuman Concentration0.59 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 62.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens CoA synthase (COASY), transcript variant 1
Alternative NameDPCK; NBIA6; NBP; pOV-2; PPAT; UKR1
Database LinkNP_079509
Entrez Gene 80347 Human
FunctionBiosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM].
Related Pathway Pantothenate and CoA biosynthesisMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COASY (RC220733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COASY.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503343)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503343)
FC Image
HEK293T cells transfected with either RC220733 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-COASY antibody(TA503343), and then analyzed by flow cytometry.

 

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