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Anti-COASY Antibody EPR8246
Also for COASY (NM_001042529)
|A synthetic peptide corresponding to residues at the C-terminus of human COASY was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, FC
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
|pH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 49% PBS, 50% Glycerol, 0.05% BSA|
|Is unsuitable for IHC-P or IP.
|Homo sapiens CoA synthase (COASY), transcript variant 2|
|DPCK; NBIA6; NBP; pOV-2; PPAT; UKR1|
Entrez Gene 80347 Human
|Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 188.8.131.52) and dephospho-CoA kinase (DPCK; EC 184.108.40.206), in prokaryotes (1,2). |
| Pantothenate and CoA biosynthesisMetabolic pathways|
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Western blot - Anti-COASY antibody [EPR8246]; All lanes : Anti-COASY antibody [EPR8246] at 1/1000 dilution.Lane 1 : HepG2 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A431 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution.Predicted band size : 62 kDa.
Other-Anti-COASY antibody [EPR8246](TA310340); Equilibrium disassociation constant (KD)..
Flow Cytometry - Anti-COASY antibody [EPR8246]; TA310340, at 1/10 dilution, staining COASY in permeabilized HeLa cells by Flow Cytometry (red) or a Rabbit IgG negative control (green).