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Anti-CLK2 PHOSPHO Antibody
Also for CLK2 (NM_001014254)
|Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 conjugated to KLH|
||0.5~1.0 mg/ml (Lot Dependent)
|100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
|Rattus norvegicus CDC-like kinase 2 (Clk2)|
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Western blot of human T47D cells showing specific immunolabeling of the ~34k cdc2 phosphorylated at Tyr15 (Control). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). The phosphospecificity of this labeling is also shown in the third and fourth lanes (lambda-phosphatase: Î»-Ptase). These blots are identical to the control except that they were incubated in Î»-Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr15 cdc2. The immunolabeling is completely eliminated by treatment with Î»-Ptase.