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Anti-CLGN GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for CLGN (NM_004362)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|Human , Rat , Monkey
|WB, ELISA, IHC
|| WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens calmegin (CLGN), transcript variant 1|
|Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility. [provided by RefSeq]|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLGN (RC205301, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLGN.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CLGN polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CLGN rabbit polyclonal antibody. (TA590180)
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.