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Home Antibody All anti-CKB antibodies

Anti-CKB Antibody EPR3926

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307503
  • Rabbit monoclonal antibody against Creatine Kinase B Type(clone EPR3926)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400689) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for CKB (NM_001823)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Creatine Kinase B type was used as an immunogen.
Clone NameEPR3926 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:50000 - 1:200000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:10 - 1:100
Predicted MW Explanation 43 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens creatine kinase, brain (CKB)
Alternative NameB-CK; BCK; CKBB; HEL-211; HEL-S-29
Database LinkNP_001814
Entrez Gene 1152 Human
Entrez Gene 12709 Mouse
Entrez Gene 24264 Rat
FunctionThe creatine kinases (CK) are a family of enzymes with highly conserved protein sequences, involved in the maintenance of ATP at sites of cellular work (1). Three cytoplasmic isoenzymes of CK are readily identified in human tissue. These isoenzymes are dimeric molecules with two dissociable subunits designated as M- (muscle) or B- (brain) type that can reassociate to form the electrophoretically distinct MM, BB, or MB isotypes (1). CK isoenzymes are crucial to energy metabolism, particularly in tissues with high energy requirements, such as skeletal muscle, heart, and brain (2). The creatine kinase B protein is a cytoplasmic enzyme involved in energy homeostasis. It reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate (3).
Related PathwayDruggable Genome Arginine and proline metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Creatine Kinase BB antibody [EPR3926]; All lanes : Anti-Creatine kinase B type antibody [EPR3926] at 1/50000 dilution.Lane 1 : 293T cell lysate.Lane 2 : SH SY5Y cell lysate.Lane 3 : Y79 cell lysate.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 43 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Creatine Kinase BB antibody [EPR3926]; TA307503, at 1/100 dilution, staining Creatine Kinase BB in Human heart by Immunohistochemistry, Paraffin-embedded tissue.
FC Image
Flow Cytometry - Anti-Creatine kinase B type antibody [EPR3926]; Overlay histogram showing SH-SY5Y cells stained with TA307503 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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