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Anti-CKB Antibody EPR3926
|A synthetic peptide corresponding to residues in human Creatine Kinase B type was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:50000 - 1:200000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens creatine kinase, brain (CKB)|
|B-CK; BCK; CKBB; HEL-211; HEL-S-29|
|The creatine kinases (CK) are a family of enzymes with highly conserved protein sequences, involved in the maintenance of ATP at sites of cellular work (1). Three cytoplasmic isoenzymes of CK are readily identified in human tissue. These isoenzymes are dimeric molecules with two dissociable subunits designated as M- (muscle) or B- (brain) type that can reassociate to form the electrophoretically distinct MM, BB, or MB isotypes (1). CK isoenzymes are crucial to energy metabolism, particularly in tissues with high energy requirements, such as skeletal muscle, heart, and brain (2). The creatine kinase B protein is a cytoplasmic enzyme involved in energy homeostasis. It reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate (3).|
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Western blot - Creatine Kinase BB antibody [EPR3926]; All lanes : Anti-Creatine kinase B type antibody [EPR3926] at 1/50000 dilution.Lane 1 : 293T cell lysate.Lane 2 : SH SY5Y cell lysate.Lane 3 : Y79 cell lysate.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 43 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Creatine Kinase BB antibody [EPR3926]; TA307503, at 1/100 dilution, staining Creatine Kinase BB in Human heart by Immunohistochemistry, Paraffin-embedded tissue.
Flow Cytometry - Anti-Creatine kinase B type antibody [EPR3926]; Overlay histogram showing SH-SY5Y cells stained with TA307503 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1Âµg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.