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Anti-CKB Antibody EPR3927
|A synthetic peptide corresponding to residues near the C-term of human Creatine Kinase B type was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||FC: 1: 10 - 100, ICC: 1:100 - 500, IHC: 1:100 - 500, WB: 1:10,000 - 50,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens creatine kinase, brain (CKB)|
|B-CK; BCK; CKBB; HEL-211; HEL-S-29|
|The creatine kinases are a family of enzymes with a highly conserved protein sequence, which are involved in the maintenance of ATP at sites of cellular work .Three cytoplasmic (non-mitochondrial) isoenzymes of creatine kinase (CK)' are readily identified in human tissue. These isoenzymes are dimeric molecules with two dissociable subunits designated as M- (muscle) or B- (brain) type that can reassociate to form the electrophoretically distinct MM, BB, or MB isotypes (1).The protein encoded by creatine kinase B is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate (2). Creatine kinase isoenzymes are crucial to energy metabolism, particularly in tissues with high energy requirements, such as skeletal muscle, heart, brain and spermatozoa (3).|
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Western blot - Anti-Creatine kinase B type antibody [EPR3927]; All lanes : Anti-Creatine kinase B type antibody [EPR3927] at 1/10000 dilution.Lane 1 : SHSY-5Y cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : Y-79 cell lysate.Lane 4 : SW840 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 43 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3927]; Paraffin embedded Human glioma tissue labelled with TA307502 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Creatine kinase B type antibody [EPR3927]; Immunofluorescence analysis of HeLa cells labelled with TA307502 at 1/100 dilution.
Flow Cytometry-Anti-Creatine kinase B type antibody [EPR3927](TA307502); Overlay histogram showing HeLa cells stained with TA307502 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.