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Anti-CHUK PHOSPHO Antibody
Also for CHUK (NM_001278)
|The antiserum was produced against synthesized phosphopeptide derived from human IKK-a around the phosphorylation site of serine 176/177 (Q-G-SP-L-C).|
|Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:1000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|IKK-. (Phospho-Ser176)/IKK-. (Phospho-Ser177) antibody detects endogenous levels of IKK-. only when phosphorylated at serine 176/177.
|Homo sapiens conserved helix-loop-helix ubiquitous kinase (CHUK)|
|IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16|
Entrez Gene 1147 Human
Entrez Gene 12675 Mouse
Entrez Gene 309361 Rat
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot analysis of extracts from 3T3 cells, treated with TNF (20ng/ml, 30mins), using IKK-a (Phospho-Ser176)/IKK-Ã?(Phospho-Ser177) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from A549 cells (Lane 2) and 3T3 cells (Lane 3), using IKK-a (Phospho-Ser176) /IKK-Ã?(Phospho-Ser177) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using IKK-a (Phospho-Ser176)/IKK-ß (Phospho-Ser177) antibody.The picture on the right is treated with the synthesized peptide.