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Home Antibody All anti-CHUK antibodies

Anti-CHUK Antibody EPR464

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307549
  • Rabbit monoclonal antibody against IKK alpha(clone EPR464)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400513) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human IKK alpha was used as an immunogen.
Clone NameEPR464 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens conserved helix-loop-helix ubiquitous kinase (CHUK)
Alternative NameIKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16
Database LinkNP_001269
Entrez Gene 1147 Human
FunctionIKK alpha (I-Kappa-B kinase alpha) is a member of the IKK complex that is composed of IKK alpha, IKK beta, IKK gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKK alpha at threonine 23 and serine 176 by AKT1 and NIK, respectively (3). It has been demonstrated that IKK alpha can accumulate in the nucleus after cytokine exposure (4). In the nucleus, IKK alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 (5).
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayChemokine signaling pathwayApoptosisToll-like receptor signaling pathwayNOD-like receptor signaling pathwayRIG-I-like receptor signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - IKK alpha antibody [EPR464]; All lanes : Anti-IKK alpha antibody [EPR464] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : Daudi cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 85 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IKK alpha antibody [EPR464]; TA307549, at 1/100 dilution, staining IKK alpha in paraffin-embedded Human breast tissue by Immunohistochemistry.

 

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