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Anti-CHUK Antibody Y466
Also for CHUK (NM_001278)
|A synthetic peptide corresponding to residues near the C-term of human IKK-beta was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent dilution; FC: 1:40; IP: 1:50
|Does not react with Rat. Is unsuitable for ICC/IF.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC/IF.
|Homo sapiens conserved helix-loop-helix ubiquitous kinase (CHUK)|
|IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16|
|This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq]. |
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot - IKK beta antibody [Y466]; Anti-IKK beta antibody [Y466] at 1/5000 dilution + Daudi cell lysate.Observed band size : 87 kDa .
Western blot - IKK beta antibody [Y466]; .Exposure time : 3 minutes
Immunohistochemistry (Paraffin-embedded sections) - IKK beta antibody [Y466]; Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using ab32135 at 1/100 dilution.
Flow Cytometry-Anti-IKK beta antibody [Y466](ab32135); Overlay histogram showing HeLa cells stained with ab32135 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.