Anti-CHEK2 Antibody, clone 8D11

Also for CHEK2 (NM_007194)

OriGene Data

Immunogen	Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.
Clone Name	clone 8D11	Isotype	IgG1
Species Reactivity	Human , Dog , Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, FC	Suggested Dilutions	WB 1:500~1000, IHC 1:50, FLOW 1:100,
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1
Alternative Name	CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
Database Link	NP_009125
Function	In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition,this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Related Pathway	Cell cycle

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CHEK2 mouse monoclonal antibody. (TA500399)

FC Image

HEK293T cells transfected with either RC201278 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CHEK2 antibody(TA500399), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-CHEK2 antibody(TA500399),(Red), compared to a nonspecific negative control antibody,(Blue).

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Anti-CHEK2 TrueMAB Antibody clone 8D11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data