Anti-CHEK2 Antibody, clone 5C4

Also for CHEK2 (NM_007194)

OriGene Data

Immunogen	Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.
Clone Name	clone 5C4	Isotype	IgG1
Species Reactivity	Human , Dog	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, IP, FC	Suggested Dilutions	WB 1:500~1000, IHC 1:150, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1
Alternative Name	CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
Database Link	NP_009125
Function	In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway	Cell cycle

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CHEK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC Image

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-CHEK2 mouse monoclonal antibody. (TA500398)

IHC Image

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-CHEK2 mouse monoclonal antibody. (TA500398)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CHEK2 mouse monoclonal antibody. (TA500398)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CHEK2 mouse monoclonal antibody. (TA500398)

IHC Image

Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-CHEK2 mouse monoclonal antibody. (TA500398)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CHEK2 mouse monoclonal antibody. (TA500398)

IF Image

Anti-CHEK2 mouse monoclonal antibody (TA500398) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHEK2(RC201278).

IF Image

Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500398).

IP Image

Immunoprecipitation(IP) of CHEK2 by using TrueMab monoclonal anti-CHEK2 antibodies (Negative control: IP without adding anti-CHEK2 antibody.). For each experiment, 500ul of DDK tagged CHEK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CHEK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-CHEK2 antibody(TA500398),(Red), compared to a nonspecific negative control antibody,(Blue).

Anti-CHEK2 TrueMAB Antibody clone 5C4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data