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Anti-CHEK2 TRUEMAB Antibody Clone OTI4C10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500385
  • CHEK2 (CHK2) mouse monoclonal antibody, clone OTI4C10 (formerly 4C10)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416128) , 20ug Explanation
100ul $325 In Stock
CF500385
  • Carrier-free (BSA/glycerol-free) CHEK2 mouse monoclonal antibody, clone OTI4C10 (formerly 4C10)
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
IP(1)
IHC(3)
FC(1)
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Also for CHEK2 (NM_007194)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.
Clone NameClone OTI4C10 IsotypeIgG2b
Species ReactivityHuman , Dog Concentration1.88 mg/ml
Guaranteed Application *WB, IHC, IP, FC Suggested DilutionsWB 1:1000~2000, IHC 1:150, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 60.7 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1
Alternative NameCDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
Database LinkNP_009125
Entrez Gene 11200 Human
Entrez Gene 486338 Dog
FunctionIn response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related PathwayProtein KinaseStem cell - PluripotencyDruggable Genome Cell cyclep53 signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CHEK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500385)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500385)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500385)
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Immunoprecipitation(IP) of CHEK2 by using TrueMab monoclonal anti-CHEK2 antibodies (Negative control: IP without adding anti-CHEK2 antibody.). For each experiment, 500ul of DDK tagged CHEK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CHEK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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HEK293T cells transfected with either RC201278 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CHEK2 antibody(TA500385), and then analyzed by flow cytometry.
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