Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1
Synonyms:
CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Threonine 68 of human Chk2 was used as immunogen. The antibody will detect Chk2 phosphorylation on Threonine 68. This antibody may also detect all splice isoforms (2-12) based on sequence h
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Chk2 is a checkpoint kinase that plays a role in regulating cell cycle progression. In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (1,2). Consisting of ionizing radiation (IR), UV irradiation or hydroxyurea treatment, DNA damage induces ATM/ATR to phosphorylate Thr68 and other sites in the amino terminal domain (3). Phosphorylation at Thr68 is a prerequisite for subsequent activation steps.
Related Pathway
Cell cycle
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Western blot analysis on 293 cell lysates using anti-Phospho-Chk2 (pT68), 1:1,000 dilution. Cells were either (A) untreated (B) treated with 2uM Doxorubicin and (C) treated with 10uM Doxorubicin.
