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Home All anti-CHEK2 antibodies
Anti-CHEK2 Antibody E126
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for CHEK2 (NM_007194)
OriGene Data
| A synthetic phospho-peptide corresponding to residues surrounding Thr68 of human Chk2 was used as immunogen. The antibody only detects Chk2 phosphorylated on Threonine 68. |
| E126 |
IgG |
| Human |
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| WB, IHC |
WB: 1: 1,000; IHC: 1: 100-1:250; ICC: 1:250-1:500; IP: 1:50 |
| 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| (Protein A or G Sepharose) |
Reference Data
| Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1 |
| CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53 |
| NP_009125 |
| Chk2 is a checkpoint kinase that plays a role in regulating cell cycle progression. In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (1,2). Consisting of ionizing radiation (IR), UV irradiation or hydroxyurea treatment, DNA damage induces ATM/ATR to phosphorylate Thr68 and other sites in the amino terminal domain (3). Phosphorylation at Thr68 is a prerequisite for subsequent activation steps. |
Cell cycle |
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
WB Image
Western blot analysis on HeLa cell lysate using anti-Chk2; dilution 1:1,000. Cells were (1) untreated, and (2) treated with Nocodazole.
IHC Image
(A) Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-Chk2; (B) IHC analysis of paraffin-embedded human breast cancer, showing nuclear localization of p53, using anti-p53
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
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