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Anti-CHEK2 Antibody E126
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Also for CHEK2 (NM_007194)
|A synthetic phospho-peptide corresponding to residues surrounding Thr68 of human Chk2 was used as immunogen. The antibody only detects Chk2 phosphorylated on Threonine 68.|
||WB: 1: 1,000; IHC: 1: 100-1:250; ICC: 1:250-1:500; IP: 1:50
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1|
|CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53|
|Chk2 is a checkpoint kinase that plays a role in regulating cell cycle progression. In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (1,2). Consisting of ionizing radiation (IR), UV irradiation or hydroxyurea treatment, DNA damage induces ATM/ATR to phosphorylate Thr68 and other sites in the amino terminal domain (3). Phosphorylation at Thr68 is a prerequisite for subsequent activation steps. |
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Western blot analysis on HeLa cell lysate using anti-Chk2; dilution 1:1,000. Cells were (1) untreated, and (2) treated with Nocodazole.
(A) Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-Chk2; (B) IHC analysis of paraffin-embedded human breast cancer, showing nuclear localization of p53, using anti-p53