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Anti-Chk1 PHOSPHO Antibody EPR915
Also for Chk1 (NM_001274)
|A phospho specific peptide corresponding to residues surrounding Serine 296 of human Chk1 was used as an immunogen. The antibody only detects Chk1 phosphorylated at Serine 296.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000; IHC 1:100 - 250; IP: 1:20;
|Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.|
|Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3|
|Chk1 is a serine/threonine-protein kinase involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can inactivate cdc25c through phosphorylation at Serine 216 (3,4 ). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).|
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Western blot - Chk1 (phospho S296) antibody [EPR915]; All lanes : Anti-Chk1 (phospho S296) [EPR915] antibody at 1/2000 dilution.Lane 1 : lysate from 293T cells, untreated.Lane 2 : lysate from 293T cells treated with UV and AP.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.Observed band size : 54 kDa.
Immunocytochemistry/ Immunofluorescence - Chk1 (phospho S296) antibody [EPR915]; Immunofluorescent staining of HeLa cells using ab79758 at 1/100 dilution.