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Anti-Chk1 PHOSPHO Antibody EPR915


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal antibody against Chk1, pS296 (Phospho-specific)
100ul $325 3-7 Days
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OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Serine 296 of human Chk1 was used as an immunogen. The antibody only detects Chk1 phosphorylated at Serine 296.
Clone NameEPR915 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1000; IHC 1:100 - 250; IP: 1:20;
BufferStore at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Reference Data

Target NameHomo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3
Alternative NameCHK1
Database LinkNP_001265
Entrez Gene 1111 Human
FunctionChk1 is a serine/threonine-protein kinase involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can inactivate cdc25c through phosphorylation at Serine 216 (3,4 ). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).
Related PathwayProtein KinaseStem cell - PluripotencyDruggable Genome Cell cyclep53 signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Chk1 (phospho S296) antibody [EPR915]; All lanes : Anti-Chk1 (phospho S296) [EPR915] antibody at 1/2000 dilution.Lane 1 : lysate from 293T cells, untreated.Lane 2 : lysate from 293T cells treated with UV and AP.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.Observed band size : 54 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Chk1 (phospho S296) antibody [EPR915]; Immunofluorescent staining of HeLa cells using TA310324 at 1/100 dilution.


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