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Home Antibody All anti-CFL1 antibodies

Anti-CFL1 PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA321717
  • Anti-CFL1 (Phospho-Ser3) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)
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Also for CFL1 (NM_005507)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens cofilin 1 (non-muscle) (CFL1)
Alternative NameCFL; HEL-S-15
Database LinkNP_005498
Entrez Gene 1072 Human
Entrez Gene 12631 Mouse
Entrez Gene 29271 Rat
FunctionControls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Related Pathway
Hemostasis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 19 kDa . Positive control: COLO205 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lane1: Treated with the peptide Lane2: COLO205 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using CFL1 (Phospho-Ser3) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
IF Image
Predicted cell location: Cytoplasm. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using CFL1 (Phospho-Ser3) antibody at dilution 1/100. (Original magnification:×200)

 

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