Home Antibody All anti-CEBPB antibodies
Also for CEBPB (NM_005194)
|This CEBPB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-76 amino acids from the N-terminal region of human CEBPB.|
|WB, IF, FC
||WB: 1:1000; IF: 1:10-50; FC: 1:10-50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Homo sapiens CCAAT/enhancer binding protein (C/EBP), beta (CEBPB), transcript variant 1|
|C/EBP-beta; CRP2; IL6DBP; LAP; LIP; NF-IL6; TCF5|
EGFR1 Signaling Pathway
Senescence and Autophagy
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Western blot analysis of CEBPB Antibody (N-term) in Hela cell line lysates (35ug/lane). CEBPB (arrow) was detected using the purified Pab.
Western blot analysis of CEBPB (arrow) using rabbit polyclonal CEBPB Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CEBPB gene (Lane 2) .
Fluorescent confocal image of Hela cell stained with CEBPB Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CEBPB primary antibody (1:25, 1 h at 37.). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37.).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37.). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CEBPB immunoreactivity is localized to nucleus significantly.
Flow cytometric analysis of hela cells using CEBPB Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.