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Anti-CEBPA Antibody EP708Y


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SKU Description Amount Price Availability*  
TA303448 Rabbit monoclonal antibody against C/EBP alpha (N-term)(EP708Y) 100ul $325 In Stock
LC418038 CEBPA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for CEBPA (NM_004364)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the N-term of human C/EBP alpha was used as immunogen. This antibody recognizes both the 42kDa and the 30kDa isoforms.
Clone NameEP708Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; ICC/IF: 1:250 - 1:500; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IHC or IP.

Reference Data

Target NameHomo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1
Alternative NameC/EBP-alpha; CEBP
Database LinkNP_004355
Entrez Gene 1050 Human
FunctionThe protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq].
Related PathwayES Cell Differentiation/IPSDruggable Genome Pathways in cancerAcute myeloid leukemia

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - CEBP Alpha antibody [EP708Y]; Anti-CEBP Alpha antibody [EP708Y] at 1/1000 dilution + U937 + LPS cell lysate at 10 ug.Predicted band size : 30,43 kDa.Observed band size : 30,43 kDa.
Western blot - Anti-CEBP Alpha antibody [EP708Y]; Anti-CEBP Alpha antibody [EP708Y] at 1/5000 dilution + Human MCF-7 whole cell lysate at 100000 cells.Secondary.HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/2000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 30,43 kDa.Observed band size : 40 kDa .Exposure time : 1 minute
Immunocytochemistry/ Immunofluorescence - CEBP Alpha antibody [EP708Y]; TA303448 (1/250) staining HeLa cells using immunofluorescence.
Flow Cytometry - CEBP Alpha antibody; Overlay histogram showing HeLa cells stained with TA303448 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.


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