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Anti-CDT1 Antibody EPR4729
Also for CDT1 (NM_030928)
|A synthetic peptide corresponding to residues near the C-terminus of human CDT1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:500 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens chromatin licensing and DNA replication factor 1 (CDT1)|
|CDT1 is a novel replication factor conserved throughout evolution, and it was first identified in S. pombe as a target of CDC10 transcriptional regulation. It has been demonstrated that CDT1 cooperates with CDC18/CDC6 in driving re-replication in G2 when the CDC18/CDC6 protein is overexpressed and that CDT1 depletion prevents the binding of MCM proteins to chromatin (1). Regulation of CDT1 plays a critical role in the licensing process. Inhibition of CDT1 activity occurs by multiple mechanisms, including ubiquitin-dependent proteolysis and binding to its potent inhibitor Geminin. Misregulation of the licensing process and defects in the ordered assembly and disassembly of the pre-RC alter the integrity of the genome. Thus, cell cycle regulation of CDT1 expression and function is responsible for licensing replication origins in G1 and preventing relicensing and hence rereplication in S phase.|
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Western blot - CDT1 antibody [EPR4729]; All lanes : Anti-CDT1 antibody [EPR4729] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : HUVEC cell lysate.Lane 4 : Jurkat cell lysate.Lane 5 : Mouse brain lysate.Lane 6 : Rat brain lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 60 kDa.
Flow Cytometry - CDT1 antibody [EPR4729]; Flow cytometric analysis of CDT1 in permeabilized Jurkat cells using ab109421 at 1/500 dilution (red) or a rabbit IgG (negative) (green).