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Home Antibody All anti-CDKN2A antibodies

Anti-CDKN2A Antibody EP4353Y(3)

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SKU Description Amount Price Availability*  
TA300862 Rabbit Monoclonal Antibody against CDKN2A (Clone EP4353Y) 100ul $325 In Stock
LC400022 CDKN2A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IF(1)
FC(1)
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Also for CDKN2A (NM_000077)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA full length p16/INK4A protein with GST tag was used as an immunogen.
Clone NameEP4353Y(3) IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:2000; ICC/IF: 1:100 - 1:500; FC: 1:50 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P or IP.

Reference Data

Target NameHomo sapiens cyclin-dependent kinase inhibitor 2A (CDKN2A), transcript variant 1
Alternative NameARF; CDK4I; CDKN2; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4;
Database LinkNP_000068
Entrez Gene 1029 Human
FunctionThe division cycle of eukaryotic cells is regulated by a family of protein kinases known as the cyclin-dependent kinases (CDKs). The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. It has been reported that p16 binds to CDK4 and inhibits the catalytic activity of the CDK4/cyclin D enzymes. p16 seems to act in a regulatory feedback circuit with CDK4, D-type cyclins and retinoblastoma protein (1). The INK4 (inhibitor of cyclin-dependent kinase 4) family consists of four tumor-suppressor proteins: p15(INK4B), p16(INK4A), p18(INK4C), and p19(INK4D). While their sequences and structures are highly homologous, they show appreciable differences in conformational flexibility, stability, and aggregation tendency (2). Cell cycle arrest at the G1 checkpoint allows completion of critical macromolecular events prior to S phase. Regulators of the G1 checkpoint include an inhibitor of cyclin-dependent kinase, p16INK4; two tumor-suppressor proteins, p53 and RB and cyclin D1. p16INK4 is a tumor-suppressor protein and that genetic and epigenetic abnormalities in genes controlling the G1 checkpoint can lead to both escape from senescence and cancer formation (3).
Related PathwayDruggable Genome Cell cyclep53 signaling pathwayPathways in cancerPancreatic cancerGliomaMelanomaMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - CDKN2A/p16INK4a antibody [EP4353Y]; All lanes : Anti-CDKN2A/p16INK4a antibody [EP4353Y(3)] at 1/20000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 17 kDa.Observed band size : 17 kDa.
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Immunocytochemistry/ Immunofluorescence - CDKN2A/p16INK4a antibody [EP4353Y]; Immunofluorescent staining of HeLa cells using 1/100 TA300862
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Flow Cytometry-Anti-CDKN2A/p16INK4a antibody(TA300862); Overlay histogram showing HEK293 cells stained with TA300862 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in HEK293 cells under the same conditions.
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