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Anti-CDK9 PHOSPHO Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA319416 Rabbit polyclonal CDK9 phospho T29 antibody (Phospho-specific) 100ug $325 2 Weeks
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OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T29 in the human CDK9 protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat Concentration1.3mg/ml
Guaranteed Application *WB Suggested DilutionsELISA: 1:5,000 - 1:24,000, WB: 1:200 - 1:2,000, IP: 1:100
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family.  CDK family members are highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2 and are known as important cell cycle regulators.  CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2.  This kinase was also found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II.  This protein forms a complex with, and is regulated by, its regulatory subunit, cyclin T or cyclin K. HIV-1 Tat protein

Reference Data

Target NameHomo sapiens cyclin-dependent kinase 9 (CDK9)
Alternative NameC-2k; CDC2L4; CTK1; PITALRE; TAK
Database LinkNP_001252
Entrez Gene 1025 Human
Entrez Gene 107951 Mouse
Entrez Gene 362110 Rat
Related PathwayProtein KinaseTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot using affinity purified anti-CDK9 pT29 antibody shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29. The primary antibody was used at a 1:1000 dilution. Personal Communication, J. Brady, NCI, Bethesda, MD.


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