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Anti-CDK7 PHOSPHO Antibody
Also for CDK7 (NM_001799)
|This CDK7 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T170 of human CDK7.|
|WB, IHC, DB
||WB: 1:1000; IHC: 1:50-100; DB: 1:500
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
|This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|Homo sapiens cyclin-dependent kinase 7 (CDK7)|
|CAK1; CDKN7; HCAK; MO15; p39MO15; STK1|
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The CDK7-T170 Pab is used in Western blot to detect CDK7-T170 in Ramos tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Dot blot analysis of Phospho-Cdk7-T170 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.