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Anti-CDK6 Antibody EPR4515
Also for CDK6 (NM_001145306)
|A synthetic peptide corresponding to residues in C-terminus of human CDK6 was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:50000 - 1:200000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:100 - 1:1000
|pH: 7.40Preservative: 0.01% Sodium azide; Constituents: 50% Glycerol, 0.05% BSA|
|Is unsuitable for IP.
|Homo sapiens cyclin-dependent kinase 6 (CDK6), transcript variant 2|
Entrez Gene 1021 Human
|CDK6 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of CDK6 is up-regulated in some types of cancer (1).|
|Protein KinaseDruggable Genome Cell cyclep53 signaling pathwayPathways in cancerPancreatic cancerGliomaMelanomaMore Pathways >> |
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Western blot - Anti-Cdk6 antibody [EPR4515]; All lanes : Anti-Cdk6 antibody [EPR4515] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : K562 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat-anti-rabbit HRP at 1/2000 dilution.Predicted band size : 37 kDa.Observed band size : 37 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk6 antibody [EPR4515]; TA310313 at 1/100 dilution, staining Cdk6 in Formalin fixed Paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 antibody [EPR4515]; ICC/IF image of TA310313 at 1/100 dilution, staining Cdk6 in HeLa cells.
Flow Cytometry - Anti-Cdk6 antibody [EPR4515]; Overlay histogram showing HeLa cells stained with TA310313 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.