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Anti-CDK2 TRUEMAB Antibody clone 2A5
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for CDK2 (NM_001798)
|Full length human recombinant protein of human CDK2 (NP_001789) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens cyclin-dependent kinase 2 (CDK2), transcript variant 1|
Entrez Gene 1017 Human
|The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq]. |
|Protein KinaseDruggable Genome Cell cycleOocyte meiosisp53 signaling pathwayProgesterone-mediated oocyte maturationPathways in cancerProstate cancerMore Pathways >> |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK2 (RC200494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK2.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CDK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502915)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502915)
Anti-CDK2 mouse monoclonal antibody (TA502915) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2(RC200494).
Immunofluorescent staining of HeLa cells using anti-CDK2 mouse monoclonal antibody (TA502915).
HEK293T cells transfected with either RC200494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CDK2 antibody(TA502915), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-CDK2 antibody(TA502915),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-CDK2 antibody(TA502915),(Red), compared to a nonspecific negative control antibody,(Blue).