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Home Antibody All anti-CDH1 antibodies

Anti-CDH1 Antibody EP700Y

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SKU Description Amount Price Availability*  
TA300622
  • Rabbit Monoclonal Antibody against CDH1 (Clone EP700Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401389) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(9)
IF(1)
FC(1)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the 5th cadherin domain of human E-cadherin was used.
Clone NameEP700Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF, FC Suggested DilutionsICC/IF: Use at an assay dependent concentration; IHC-P: 1:500; ICC: 1:500; IP: 1:50; FC: 1:1000; WB: 1:10000 - 1:50000
Predicted MW Explanation 97 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens cadherin 1, type 1, E-cadherin (epithelial) (CDH1)
Alternative NameArc-1; CD324; CDHE; ECAD; LCAM; UVO
Database LinkNP_004351
Entrez Gene 999 Human
FunctionE-Cadherin, a member of the cadherin superfamily, is implicated as a key player in different cellular processes including development, morphology, polarity, migration and tissue integrity (1-2). E-cadherin is a glycoprotein with an extracellular domain that interacts with other E-cadherin molecules on adjacent cells, thereby establishing adhesion between epithelial cells. Established as a tumor and metastasis suppressor, E-cadherin expression is frequently down-regulated or extinguished in malignancy which strongly correlates with poor prognosis (3-4). Mutations in E-cadherin are found in gastric, breast, colorectal, thyroid and ovarian cancer (5).
Related PathwayES Cell Differentiation/IPSTransmembraneDruggable Genome Cell adhesion molecules (CAMs)Adherens junctionPathogenic Escherichia coli infectionPathways in cancerEndometrial cancerThyroid cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-E Cadherin antibody [EP700Y]; All lanes : Anti-E Cadherin [EP700Y] antibody at 1/10000 dilution.Lane 1 : MCF7 cell lysate (positive control).Lane 2 : MDA-MB-231 cell lysate (negative control).Lysates/proteins at 100000 cells per lane.Secondary.Goat anti-rabbit IgG HRP.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 97 kDa.Observed band size : 100-150 kDa .Exposure time : 5 minutes
Assay Image
Other-Anti-E Cadherin antibody [EP700Y](TA300622); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - E Cadherin antibody [EP700Y]; Paraffin-embedded human breast carcinoma.TA300622 at 1/500 dilution.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - E Cadherin antibody [EP700Y]; Paraffin-embedded human hepatocellular carcinoma.TA300622 at 1/500 dilution
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody [EP700Y]; TA300622 stainingE Cadherinin humankidneytissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation and then blockingwith 10% serum was performedfor1 hourat 25°C. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 25°C. ABiotin conjugatedgoat polyclonal to rabbit IgG was used at dilution at 1/400 as secondary antibody.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y]; TA300622 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y]; TA300622 showing positive staining in Colonic adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y]; TA300622 showing positive staining in Papillary carcinoma of thyroid gland tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y]; TA300622 showing positive staining in Transitional cell carcinoma of kidney tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y]; TA300622 showing positive staining in Lung adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y]; Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using TA300622. Green-E-Cadherin red-PI.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EP700Y]; ICC/IF image of TA300622 stained human MCF7 cells. The cells were fixed in Paraformaldehyde, before incubation in 0.1% BSA + 1% goat serum in PBS as a blocking agent for 30 mins at 22oC. The cells were then incubated with the antibody for 2 hours at 22°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG. DAPI was used to stain the cell nuclei (blue).
FC Image
Flow Cytometry - Anti-E Cadherin antibody [EP700Y]; Overlay histogram showing A431 cells stained with TA300622 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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