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Anti-CDC25C Antibody E302
Also for CDC25C (NM_001790)
|A synthetic peptide corresponding to residues near N-terminus of human Cdc25C was used as immunogen. The antibody can also detect splice isoform 2, 4 and 5 of human Cdc25C, based on sequence homology.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:5000; IHC-P: 1:250 - 1:500; ICC/IF: 1:250; FC: Use at an assay dependent dilution; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse, Rat
|Homo sapiens cell division cycle 25C (CDC25C), transcript variant 1|
|This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq]. |
Wnt Signaling Pathway
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Western blot - Cdc25C antibody [E302]; Anti-Cdc25C antibody [E302] at 1/5000 dilution + HeLa cell lysate.Predicted band size : 53 kDa.Observed band size : 60 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Cdc25C antibody [E302]; Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma ab32444 at 1/250 dilution.
Flow Cytometry - Anti-Cdc25C antibody [E302]; Flow cytometry analysis of HeLa cells, staining Cdc25C with ab32444. . Cells were fixed with formaldehyde and permeabilized with 90% methanol. Samples were incubated with primary antibody (1/20 in PBS + 10% goat serum) for 1 hour at 23°C. A FITC-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.