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Anti-CDC25C PHOSPHO Antibody E190
Also for CDC25C (NM_001790)
|A synthetic phospho-peptide corresponding to residues surrounding Ser216 of human Cdc25C was used as immunogen. The antibody only detects Cdc25C phosphorylated on Serine 216.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1,000; ICC: 1:50; IP: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens cell division cycle 25C (CDC25C), transcript variant 1|
|This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq]. |
Wnt Signaling Pathway
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Western blot - Anti-Cdc25C (phospho S216) antibody [E190]; All lanes : Anti-Cdc25C (phospho S216) antibody [E190] at 1/1000 dilution.Lane 1 : IR treated HeLa cell lysate.Lane 2 : 10 Gy IR treated (0 minutes recovery) HeLa cell lysate.Lane 3 : 10 Gy IR treated (30 minutes recovery) HeLa cell lysate.Lane 4 : 10 Gy IR treated (60 minutes recovery) HeLa cell lysate.Lysates/proteins at 22 µg per lane.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 53 kDa.Exposure time : 5 secondsThis image is courtesy of an anonymous Abreview
Western blot - Cdc25C (phospho S216) antibody [E190]; Lane 1 : Anti-Cdc25C (phospho S216) antibody [E190] at 1/1000 dilution (Untreated ).Lane 2 : Anti-Cdc25C (phospho S216) antibody [E190] at 1/1000 dilution (Treated with phosphatase).Lane 1 : As above.Lane 2 : As above.Predicted band size : 53 kDa.Observed band size : 53,60 kDa .