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Anti-CDC2 Antibody E161
Also for CDC2 (NM_001786)
|A synthetic peptide corresponding to residues surrounding Tyr15 of human Cdc2 was used as immunogen. The antibody detects Cdc2 without phosphorylation on Tyrosine 15. It does not detect Y15-phosphorylated Cdc2. Predicted to cross-react with bovine, based|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IHC-P: 1:50; ICC: 1:500; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt.
|Homo sapiens cyclin-dependent kinase 1 (CDK1), transcript variant 1|
|CDC2; CDC28A; P34CDC2|
|During the eukaryotic cell cycle, activation of Cdc2 (Cdk1) kinase regulates the transition into M-phase (1). Cdc2 forms a complex with cyclin B, and activation of this complex is controlled by dephosphorylation of Cdc2 at tyrosine 15 and threonine 14 (2). Phosphorylation at Tyr15 and is carried out by Wee1 and Myt1 protein kinases (3), while Tyr15 dephosphorylation is carried out by the cdc25 phosphatase (4).|
Hedgehog signaling pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - Anti-CDK1 antibody [E161]; All lanes : Anti-CDK1 antibody [E161] at 1/1000 dilution.Lane 1 : A431 cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : SKBR3 cell lysate.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 34 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Cdc2 antibody [E161]; Ab32384, at a 1/50 dilution, staining Cdc2 in paraffin embedded human lymphoma tissue sections by Immunohistochemistry.