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Home Antibody All anti-CDC16 antibodies

Anti-CDC16 PHOSPHO Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA319342 Rabbit polyclonal APC6 phospho T580 antibody (Phospho-specific) 100ug $325 3-7 Days
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Also for CDC16 (NM_003903)
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OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 575-588 of Human Apc6 protein.
Clone Name IsotypeIgG
Species Reactivityhuman Concentration0.97 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:1,000 - 1:10,000, WB: 1:500 - 1:3,000, IF: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note APC6 (also known as Cell division cycle protein 16 homolog (CDC16Hs), anaphase promoting complex subunit 6, and cyclosome subunit 6) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle.   The APC/C is composed of at least 11 subunits.   APC6 interacts with PPP5C and CDC20. This protein colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.  Splice variants have been described for this protein.

Reference Data

Target NameHomo sapiens cell division cycle 16 (CDC16), transcript variant 1
Alternative NameANAPC6; APC6; CUT9
Database LinkNP_003894
Entrez Gene 8881 Human
Related PathwayDruggable Genome Cell cycleOocyte meiosisUbiquitin mediated proteolysisProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
WB using Anti-APC6 pT580 antibody shows detection of a band ~72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35ug of lysates. Primary antibody diluted to 1:1,000 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX .


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