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Home Antibody All anti-CD99 antibodies

Anti-CD99 Antibody EPR3096

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307595
  • Rabbit monoclonal antibody against CD99(clone EPR3096)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC426583) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(4)
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Also for CD99 (NM_001122898)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to the extracellular domains in human CD99 was used as an immunogen.
Clone NameEPR3096 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC: 1:250 - 1:500; FC: 1:10 - 1:100
Predicted MW Explanation 19 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens CD99 molecule (CD99), transcript variant 2
Alternative NameHBA71; MIC2; MIC2X; MIC2Y; MSK5X
Database LinkNP_001116370
Entrez Gene 4267 Human
FunctionCluster of Differentiation 99 (CD99), also known as MIC2, is a T-cell surface glycoprotein involved in the T-cell adhesion process and spontaneous rosette formation with erythrocytes (1). It functions in the migration of leukocytes and the transport of ganglioside GM1 and transmembrane proteins (2). CD99 is also implicated in other cellular events such as thymocyte selection, apoptosis of neuronal cells, and T cell activation (3). Two isoforms of CD99 exist. The expression of the full-length long form (32 kDa) promotes cell adhesion, whereas the truncated short form (28 kDa) inhibits the process (3). The short-form isoform favors migration and metastasis of osteosarcoma, while the long-form version dramatically inhibits progression (3). Moreover, CD99 is used as an immunohistochemical marker for Ewing sarcoma and other neuroectodermal tumors (4).
Related PathwayTransmembraneDruggable Genome Cell adhesion molecules (CAMs)Leukocyte transendothelial migration

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - CD99 antibody [EPR3096]; All lanes : Anti-CD99 antibody [EPR3096] at 1/1000 dilution.Lane 1 : THP-1 cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : U937 cell lysate.Lane 4 : Molt-4 cell lysate.Lane 5 : HuT-78 cell lysate.Lane 6 : HUVEC cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 19 kDa.Observed band size : 28,32 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD99 antibody [EPR3096]; Immunohistochemical staining of paraffin-embedded Human pancreas tissue using TA307595 at a dilution of 1/250.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD99 antibody [EPR3096]; Immunohistochemical staining of paraffin-embedded Human tonsil tissue using TA307595 at a dilution of 1/250.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD99 antibody [EPR3096]; Immunohistochemical staining of paraffin-embedded Human uterus tissue using TA307595 at a dilution of 1/250.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD99 antibody [EPR3096](TA307595); Fluorescent immunohistochemical analysis of paraffin-embedded human normal pancreas tissue using TA307595. Green-CD99 red-PI.

 

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