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Anti-CD86 Antibody EP1158Y
Also for CD86 (NM_175862)
|A synthetic peptide corresponding to residues near the N-terminus of human B7-2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:5000; IP: 1:50; ICC: 1:100 - 1:250; FC: 1:10; IHC-P: Use at an assay dependent dilution; IHC-Fr: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens CD86 molecule (CD86), transcript variant 1|
|B7-2; B7.2; B70; CD28LG2; LAB72|
|B7-2 (CD86) is an important costimulatory molecule for the priming and activation of naive and memory T cells, respectively. Soluble CD86 is produced by resting monocytes and results from an alternatively spliced transcript (CD86deltaTM) characterized by deletion of the transmembrane domain (1). Engagement of CD28 with B7-1 and B7-2 ligands on antigen-presenting cells (APCs) provides a stimulatory signal for T-cell activation, whereas subsequent engagement of CTLA-4 with these same ligands results in attenuation of the response. Given their central function in immune modulation, CTLA-4- and CD28-associated signaling pathways are primary therapeutic targets for preventing autoimmune disease, graft versus host disease, graft rejection and promoting tumor immunity (2). It has also been shown that on B cells and macrophages, heat shock proteins GroES and GroEL both stimulated the expression of B7-2 (3) |
Toll-like receptor signaling pathway
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Western blot - CD86 antibody [EP1158Y]; Anti-CD86 [EP1158Y] antibody at 1/5000 dilution + Raji cell lysate at 10 µg.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Observed band size : 70 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 [EP1158Y] antibody; ab53004 stainingCD86 inCynomolgus Monkeylymph nodetissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/800) for30 minutesat room temperature. An undilutedBiotin-conjugatedGoat anti-rabbit IgG polyclonalwas used as the secondary antibody.
Immunohistochemistry (Paraffin-embedded sections) - CD86 antibody [EP1158Y]; ab53004, at a 1/100 dilution, staining Human CD86 in Tonsil, using Immunohistochemistry, Paraffin embedded tissue.
Flow Cytometry - Anti-CD86 antibody [EP1158Y]; Overlay histogram showing Raji cells stained with ab53004 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Raji cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.