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Anti-CD81 Antibody EPR4244
Also for CD81 (NM_004356)
|A synthetic peptide corresponding to residues in human CD81 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; ICC: 1:100 - 1:250; FC: 1:100 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens CD81 molecule (CD81)|
|CVID6; S5.7; TAPA1; TSPAN28|
|CD81 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. CD81 mediates signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. It is localized in the tumor-suppressor gene region, and thus it is a candidate for malignancies.|
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Western blot - TAPA1 antibody [EPR4244]; All lanes : Anti-TAPA1 antibody [EPR4244] at 1/1000 dilution.Lane 1 : Ramos cell lysate.Lane 2 : JAR cell lysate.Lane 3 : U87 MG cell lysate.Lane 4 : IM 9 cell lysate.Lane 5 : Peripheral blood mononuclear cell (PBMC) cell lysate.Lane 6 : C6 cell lysate.Lane 7 : PC 12 cell lysate.Lane 8 : RAW264.7 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 26 kDa.
Flow Cytometry - Anti-TAPA1 antibody [EPR4244]; Overlay histogram showing Jurkat cells stained with ab109201 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.