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Anti-CD47 Antibody EPR4150(2)
Also for CD47 (NM_001025079)
|A synthetic peptide corresponding to residues in the intracellular domain in human CD47 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; ICC: 1:100 - 1:250; FC: 1:100 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens CD47 molecule (CD47), transcript variant 3|
|IAP; MER6; OA3|
|CD47 is a membrane protein that is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to the extracellular matrix. It is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, and it is reduced in expression on Rh erythrocytes (1).|
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Western blot - CD47 antibody [EPR4150(2)]; All lanes : Anti-CD47 antibody [EPR4150(2)] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 35 kDa.
Flow Cytometry - CD47 antibody [EPR4150(2)]; Flow cytometric analysis of CD47 in permeabilized Jurkat cells using ab108415 at 1/100 dilution (red) or a rabbit IgG (negative) (green).