OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-CD4 antibodies

Anti-CD4 Antibody EPR6855

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310278
  • Rabbit Monoclonal antibody against CD4
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400209) , 20ug
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(6)
FC(1)
spacer
Also for CD4 (NM_000616)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA recombinant protein fragment corresponding to residues in human CD4 was used as an immunogen.
Clone NameEPR6855 IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens CD4 molecule (CD4), transcript variant 1
Alternative NameCD4mut
Database LinkNP_000607
FunctionT-cell surface glycoprotein CD4 (CD4) is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It regulates T-cell activation, partially by recruiting the tyrosine kinase Lck, and induces the aggregation of lipid rafts. CD4 binds to HIV-1 gp120 and to P4HB/PDI, and upon HIV-1 binding to the cell membrane, is part of the P4HB/PDI-CD4-CXCR4-gp120 complex (1).
Related Pathway
Hematopoietic cell lineage

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-CD4 antibody [EPR6855]; All lanes : Anti-CD4 antibody [EPR6855] at 1/1000 dilution.Lane 1 : THP1 cell lysate.Lane 2 : Human fetal thymus lysate.Lane 3 : Human tonsil lysate.Lane 4 : Human lymph node lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 51 kDa.Observed band size : 51 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855]; Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD4 with TA310278 at 1/100 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855]; Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling CD4 with TA310278 at 1/100 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD4 antibody [EPR6855](TA310278); TA310278 showing positive staining in Normal liver tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD4 antibody [EPR6855](TA310278); TA310278 showing positive staining in Normal spleen tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD4 antibody [EPR6855](TA310278); TA310278 showing positive staining in Normal colon tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD4 antibody [EPR6855](TA310278); TA310278 showing positive staining in Thymoma tissue.
FC Image
Flow Cytometry - Anti-CD4 antibody [EPR6855]; Human peripheral blood lymphocytes stained with TA310278 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody for 30 min at 4°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title