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CD3E UltraMAB Antibody, Clone UMAB54
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for CD3E (NM_000733)
|Full length human recombinant protein of human CD3E(NP_000724) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||IHC 1:100, IF 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens CD3e molecule (CD3E)|
|IMD18; T3E; TCRE|
|The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]. |
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CD3E monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-UM500048 mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UM500048 mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-UM500048mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IHC staining of FFPE human tonsil using anti-CD3E clone UMAB48 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB. UM500048 requires heat-induced epitope retrieval with Citrate pH6.O. The image shows strong membranous and cytoplasmic staining.
Immunofluorescent staining of Jurkat cells using CD3E mouse monoclonal antibody (UM500048, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.