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Anti-CD247 PHOSPHO Antibody EP265(2)Y
Also for CD247 (NM_000734)
|A phospho specific peptide corresponding to residues surrounding tyrosine 142 of human CD3 zeta was used as an immunogen. This antibody detects CD3 zeta phosphorylated on tyrosine 142.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; ICC: 1:250; IP: 1:50
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens CD247 molecule (CD247), transcript variant 2|
|CD3-ZETA; CD3H; CD3Q; CD3Z; T3Z; TCRZ|
|CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR) (1). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C (1,2). CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways (3). Loss of the zeta chain results in the synthesis of unstable TCRs (4). The reversible phosphorylation of tyrosine residues is an important mechanism for modulating biological processes such as cellular signaling, differentiation, and growth, and if deregulated, can result in various types of cancer (5). |
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Western blot - CD3 zeta (phospho Y142) antibody [EP265(2)Y]; All lanes : Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] at 1/2000 dilution.Lane 1 : Jurkat cell lysates, membrane un-treated.Lane 2 : Jurkat cell lysates, membrane treated with pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 19 kDa.Observed band size : 19 kDa.