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CD2 UltraMAB Antibody, Clone UMAB6
UltraMAB Antibodies - Validated with 10K Protein Chip
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|Protein expressed in 293T cell transfected with human CD2 expression vector|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, 10K-CHIP
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens CD2 molecule (CD2)|
|LFA-2; SRBC; T11|
|CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function|
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD2 monoclonal antibody (Clone UMAB6).
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CD2 mouse monoclonal antibody. (Clone UMAB6, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD2 mouse monoclonal antibody. (Clone UMAB6, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IHC staining of paraffin-embedded human tonsil using anti-CD2 clone UMAB6 mouse monoclonal antibody at 1:200 of 0.6mg/mL and detection with Polink2 Broad HRP DAB. UM500006 requires heat-induced epitope retrieval with Accel pH8.7 at 95-100C 30 minutes [do not let boil] or 10 min in pressure cooker. The image shows strong membranous and cytoplasmic staining in >50 % of non germinal center cells of tonsil and <20% of the germinal center cells. No staining was seen in the squamous epithelia cells.
Immunofluorescent staining of Jurkat cells using anti-CD2 mouse monoclonal antibody (UM500006, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 8µm.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CD2 mouse monoclonal antibody (Clone UMAB6). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-CD2 (Clone UMAB6) very specifically recognizes Cd2 antigen on OriGene protein microarray chip.