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Home Antibody All anti-CD14 antibodies

Anti-CD14 Antibody EPR3653

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307685 Rabbit monoclonal antibody against CD14 (clone EPR3653 ) 100ul $325 In Stock
LC424624 CD14 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(2)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human CD14 was used as an immunogen.
Clone NameEPR3653 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, Assay, IHC Suggested DilutionsWB: 1:1000 - 1:5000; IHC-P: 1:500 - 1:1000; ICC: 1:100 - 1:250
BufferpH: 7.40Preservative: 0.01% Sodium azide; Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens CD14 molecule (CD14), transcript variant 1
Alternative NameCD14 antigen; monocyte differentiation antigen CD14; CD14 molecule
Database LinkNP_000582
Entrez Gene 929 Human
FunctionCD14 is a glycophosphatidylinositol-anchored protein expressed on the surfaces of monocytes and plymorphonuclear leukocytes. It acts as a co-receptor for the detection of bacterial lipopolysaccharide (LPS) (1). CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial LPS. It acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (2). CD14 is expressed strongly on peripheral blood monocytes and macrophages, and weakly on the surface of granulocytes (1).
Related PathwayES Cell Differentiation/IPSAdult stem cellsDruggable Genome MAPK signaling pathwayToll-like receptor signaling pathwayHematopoietic cell lineageRegulation of actin cytoskeletonPathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-CD14 antibody [EPR3653]; All lanes : Anti-CD14 antibody [EPR3653] at 1/1000 dilution.Lane 1 : PBMC cell lysate.Lane 2 : Human tonsil cell lysate.Lane 3 : SW480 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat Anti-rabbit HRP at 1/2000 dilution.Predicted band size : 40 kDa.Observed band size : 53 kDa .
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Other-Anti-CD14 antibody [EPR3653](TA307685); Equilibrium disassociation constant (KD)..
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653]; Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human placenta tissue labelling CD14 with TA307685 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653]; Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human tonsil tissue labelling CD14 with TA307685 at 1/500 dilution.
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