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Home Antibody All anti-CD14 antibodies

Anti-CD14 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302271
  • Rabbit Polyclonal Antibody against CD14 (N-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424624) , 20ug Explanation
100ug 325 3-7 Days
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WB(2)
IHC(1)
IF(1)
FC(1)
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Also for CD14 (NM_000591)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CD14.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000, IHC: 1:10~50, IF: 1:10~50, FC: 1:10~50
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens CD14 molecule (CD14), transcript variant 1
Alternative NameCD14 antigen; monocyte differentiation antigen CD14; CD14 molecule
Database LinkNP_000582
Entrez Gene 929 Human
FunctionCD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.
Related PathwayES Cell Differentiation/IPSAdult stem cellsDruggable Genome MAPK signaling pathwayToll-like receptor signaling pathwayHematopoietic cell lineageRegulation of actin cytoskeletonPathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (N-term) (Cat.#TA302271). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).
WB Image
CD14 Antibody (N-term) (Cat.#TA302271) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow).
IHC Image
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (N-term) (Cat.#TA302271), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF Image
Confocal immunofluorescent analysis of CD14 Antibody (N-term)(Cat#TA302271) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
FC Image
CD14 Antibody (N-term) (Cat. #TA302271) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

 

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