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Anti-CCT2 Antibody EPR4084
Also for CCT2 (NM_006431)
|A synthetic peptide corresponding to residues on the C-terminus in human TCP1 Beta was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||FC: 1: 10 - 100, ICC: 1: 100 - 250, IHC: 1: 100 - 250, IP: 1: 10 - 100, WB: 1:10,000-50,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens chaperonin containing TCP1, subunit 2 (beta) (CCT2), transcript variant 1|
|99D8.1; CCT-beta; CCTB; HEL-S-100n; PRO1633; TCP-1-beta|
|T-complex polypeptide 1 subunit beta (TCP1 beta) is a cytosolic molecular chaperone that belongs to the chaperonin-containing TCP1 (CCT) complex (1). The CCT complex is composed of eight subunits that assist in the folding of actin, tubulin, and other cytosolic proteins (1). The sequence differences among the CCT subunits are located mainly in their apical domains, suggesting some degree of specificity towards substrate binding (2). TCP1 beta plays a role in the folding of newly translated proteins in the cytosol (3). Major cytoskeletal proteins such as actin and tubulin, depend on CCT to maintain the integrity of the microfilament and microtubule-based cytoskeletal systems (4). The beta-tubulin/TCP1 beta complex may serve as an effective chemotherapeutic target for treating tumors overexpressing TCP1 beta (5).|
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Western blot - TCP1 beta antibody [EPR4084]; All lanes : Anti-TCP1 beta antibody [EPR4084] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : MCF-7 cell lysate.Lane 3 : Daudi cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 57 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCP1 beta antibody [EPR4084]; TA307158, at a 1/100 dilution, staining TCP1 beta in formalin fixed, paraffin embedded (1) Human colon tissue and (2) Human kidney tissue by Immunohistochemistry. Detection: DAB staining.
Immunocytochemistry/ Immunofluorescence - TCP1 beta antibody [EPR4084]; TA307158, at a 1/100 dilution, staining TCP1 beta in HeLa cells by Immunofluorescence.
Flow Cytometry-Anti-TCP1 beta antibody [EPR4084](TA307158); Overlay histogram showing HeLa cells stained with TA307158 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.