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Anti-CCNE2 Antibody E142
Also for CCNE2 (NM_057749)
|A synthetic peptide corresponding to residues in C-terminus of human Cyclin E2 was used as immunogen. Predicted to cross-react with mouse and bovine, based on sequence homology.|
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:500; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; FC: 1:20
|Does not react with Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for IP.
|Homo sapiens cyclin E2 (CCNE2)|
|Cyclin E2 is a member of the cyclin E family that can associate with and activate Cdk2. Expression of cyclin E2 is essential for the control of the cell cycle at the late G1 and early S phase, and this protein may regulate distinct rate-limiting pathway(s) during this phase (1). Cyclin E2 specifically interacts with Cdk inhibitors of the CIP/KIP family and activates both Cdk2 and Cdk3. Expression of the viral E6 oncoprotein in normal human fibroblasts increases levels of cyclin E2, but not cyclin E1, while expression of the E7 oncoprotein upregulates both (2). Abnormally high levels of cyclin E expression have frequently been observed in human cancers (3). |
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Western blot - Cyclin E2 antibody [E142]; Anti-Cyclin E2 antibody [E142] at 1/500 dilution + HeLa cell lysate.Predicted band size : 45 kDa.Observed band size : 50 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Cyclin E2 antibody [E142]; Immunohistochemical analysis of Cyclin E2 expression in paraffin embedded human breast carcinoma using 1/50 TA300304.
Immunocytochemistry/ Immunofluorescence - Cyclin E2 antibody [E142]; Immunofluorescent analysis of Cyclin E2 expression in HeLa cell culture using 1/100 TA300304.
Flow Cytometry-Anti-Cyclin E2 antibody [E142](TA300304); Overlay histogram showing HeLa cells stained with TA300304 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.