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Home Antibody All anti-CCNE2 antibodies

Anti-CCNE2 Antibody E142

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300304
  • Rabbit Monoclonal Antibody against CCNE2 (Clone E142)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC403299) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in C-terminus of human Cyclin E2 was used as immunogen. Predicted to cross-react with mouse and bovine, based on sequence homology.
Clone NameE142 IsotypeIgG fraction
Species ReactivityHuman, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:500; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; FC: 1:20
BufferDoes not react with Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens cyclin E2 (CCNE2)
Alternative NameCYCE2
Database LinkNP_477097
FunctionCyclin E2 is a member of the cyclin E family that can associate with and activate Cdk2. Expression of cyclin E2 is essential for the control of the cell cycle at the late G1 and early S phase, and this protein may regulate distinct rate-limiting pathway(s) during this phase (1). Cyclin E2 specifically interacts with Cdk inhibitors of the CIP/KIP family and activates both Cdk2 and Cdk3. Expression of the viral E6 oncoprotein in normal human fibroblasts increases levels of cyclin E2, but not cyclin E1, while expression of the E7 oncoprotein upregulates both (2). Abnormally high levels of cyclin E expression have frequently been observed in human cancers (3).
Related Pathway
Cell cycle

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WB Image
Western blot - Cyclin E2 antibody [E142]; Anti-Cyclin E2 antibody [E142] at 1/500 dilution + HeLa cell lysate.Predicted band size : 45 kDa.Observed band size : 50 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Cyclin E2 antibody [E142]; Immunohistochemical analysis of Cyclin E2 expression in paraffin embedded human breast carcinoma using 1/50 TA300304.
IF Image
Immunocytochemistry/ Immunofluorescence - Cyclin E2 antibody [E142]; Immunofluorescent analysis of Cyclin E2 expression in HeLa cell culture using 1/100 TA300304.
FC Image
Flow Cytometry-Anti-Cyclin E2 antibody [E142](TA300304); Overlay histogram showing HeLa cells stained with TA300304 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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