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Home Antibody All anti-CCNB1 antibodies

Anti-CCNB1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319229
  • Rabbit polyclonal Cyclin B1 antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC410417) , 20ug
100ul $325 3-7 Days
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WB(2)
IHC(1)

OriGene Data

ImmunogenAnti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.
Clone Name IsotypeIgG
Species Reactivityhuman, rat, mouse Concentration80 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:1,000, IP: 1:100
BufferNone
Note The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Reference Data

Target NameHomo sapiens cyclin B1 (CCNB1)
Alternative NameCCNB
Database LinkNP_114172
Function
Related Pathway
Cell cycle
Hedgehog signaling pathway

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WB Image
Western blot analysis using anti-Cyclin B1 antibody shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B1 (arrowhead). Approximately 50 µg of lysate was loaded on to a 7% SDS-PAGE gel for separation. After transfer to nitrocellulose, the blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG (p/n 611-103-122). Personal communication, Luca D'Agostino, Temple University, Philadelphia, PA.
WB Image
Western blot analysis using anti-cyclin B<sub>1</sub> antibody shows detection of human Cyclin B<sub>1</sub> present in asynchronous HN30 cell lysates.  HN30 cells are from head and neck cancer tumors that over express cyclin B<sub>1</sub> and D<sub>1</sub>. Comparison to a molecular weight marker indicates a band of ~62 kDa corresponding to the expected molecular weight for the protein (arrowhead).  The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature.  Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG 611-103-122 and chemiluminescence reagent with a 1-min exposure time.  Other detection systems will yield similar results. Personal communication, Luca Cote, Temple University, Philadelphia, PA.
IHC Image
anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

 

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