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Also for CCNB1 (NM_031966)
|The antiserum was produced against synthesized non-phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-S-P-S).|
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:20000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|Cyclin B1 (Ab-126) antibody detects endogenous levels of total Cyclin B1 protein.
|Homo sapiens cyclin B1 (CCNB1)|
Hedgehog signaling pathway
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Western blot analysis of extracts from HeLa cells, treated with serum (10%, 15min), using Cyclin B1 (Ab-126) antibody (#BOO68, Line 1 and 2).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (Ab-126) antibody (#BOO68).The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using Cyclin B1 (Ab-126) antibody (#BOO68).The picture on the right is treated with the synthesized peptide.