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Anti-CCNB1 PHOSPHO Antibody EPR3670
Also for CCNB1 (NM_031966)
|A phospho specific peptide corresponding to residues surrounding Serine 126 of human Cyclin B1 was used as an immunogen. This antibody only detects Cyclin B1 phosphorylated at Serine 126.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC/IF or IHC-P.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
|Homo sapiens cyclin B1 (CCNB1)|
|Cyclin B1 regulates mitosis in conjunction with Cdc2 kinase to form the Cdc2-cyclin B1 complex also known as maturation promoting factor (MPF) (1-2). The activation of the complex begins when the regulatory subunit, cyclin B1, associates with the catalytic subunit of Cdc2. Cyclin B1 levels rise during S phase and G2, and peak at mitosis. The timing of mitosis can also be influenced by changes in cyclin B1Cdc2 localization. Cyclin B1 is mostly located in the cytoplasm during S and G2 phases and then rapidly accumulates in the nucleus at the onset of prophase (3).|
Hedgehog signaling pathway
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Western blot - Anti-Cyclin B1 (phospho S126) antibody [EPR3670]; All lanes : Anti-Cyclin B1 (phospho S126) antibody [EPR3670] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Lysate of HeLa cells treated with Nocodazole .Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution.Predicted band size : 48 kDa.