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Also for CCL2 (NM_002982)
|This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human MCP-1 protein produced in E.coli.|
||ELISA: 1:10,000, WB: 1:1000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|MCP-1, or Monocyte chemoattractant protein 1, of the intercrine beta (chemokine CC) family, is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. It augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. MCP-1 exists as a monomer or homodimer; in equilibrium. It binds to CCR2 and CCR4 and is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
|Homo sapiens chemokine (C-C motif) ligand 2 (CCL2)|
|GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF|
Cytokine-cytokine receptor interaction
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Anti-human MCP-1 by western blot shows detection of recombinant human MCP-1 raised in E.coli. Recombinant (0.1 µg, 8.6 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Rockland's, Inc. Anti-Human MCP-1. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (p/n 611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.