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Home Antibody All anti-CAST antibodies

Anti-CAST TRUEMAB Antibody Clone 1E7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA504351
  • CAST (Calpastatin) mouse monoclonal antibody, Clone 1E7
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400663) , 20ug
100ul 379 In Stock
CF504351
  • Carrier-free (BSA/glycerol-free) CAST mouse monoclonal antibody,clone 1E7
100ug $450 3-4 weeks
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WB(1)
IHC(5)
FC(1)
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Also for CAST (NM_001750)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human CAST(NP_001741) produced in HEK293T cell.
Clone NameClone 1E7 IsotypeIgG1
Species ReactivityHuman Concentration0.62 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens calpastatin (CAST), transcript variant 1
Alternative NameBS-17; MGC9402; MGC9402; OTTHUMP00000158519; OTTHUMP00000158520; calpain inhibitor; heart-type calpastatin; sperm BS-17 component; calpastatin
Database LinkNP_001741
FunctionThe protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAST (RC211776, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAST.
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504351)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504351)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504351)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504351)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504351)
FC Image
HEK293T cells transfected with either RC211776 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CAST antibody(TA504351), and then analyzed by flow cytometry.

 

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