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Home Antibody All anti-CAST antibodies

Anti-CAST TRUEMAB Antibody Clone OTI1E7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA504351 CAST (Calpastatin) mouse monoclonal antibody, clone OTI1E7 (formerly 1E7) 100ul $379 In Stock
LC400663 CAST HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF504351 Carrier-free (BSA/glycerol-free) CAST mouse monoclonal antibody, clone OTI1E7 (formerly 1E7) 100ug $450 3-4 weeks
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WB(1)
IHC(5)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human CAST(NP_001741) produced in HEK293T cell.
Clone NameClone OTI1E7 IsotypeIgG1
Species ReactivityHuman Concentration0.62 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 84.8 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens calpastatin (CAST), transcript variant 1
Alternative NameBS-17; MGC9402; MGC9402; OTTHUMP00000158519; OTTHUMP00000158520; calpain inhibitor; heart-type calpastatin; sperm BS-17 component; calpastatin
Database LinkNP_001741
Entrez Gene 831 Human
FunctionThe protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAST (RC211776, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAST.
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504351)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504351)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504351)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504351)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504351)
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HEK293T cells transfected with either RC211776 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CAST antibody(TA504351), and then analyzed by flow cytometry.
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