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Also for CASP9 (NM_032996)
|The antiserum was produced against synthesized non-phosphopeptide derived from human Caspase 9 around the phosphorylation site of tyrosine 153 (L-A-YP-I-L).|
||WB: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:5000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|Caspase 9 (Ab-153) antibody detects endogenous levels of total Caspase 9 protein.
|Homo sapiens caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant 3|
|APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56|
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot analysis of extracts from HeLa cells, treated with EGF (200ng/ml, 30mins), using Caspase 9 (Ab-153) antibody.The lane on the right is treated with the synthesized peptide.Western blot analysis of extracts from 3T3 cells (Lane 2), using B-Myb (Ab-494/497) Antibody. The lane on the left is treated with synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Caspase 9 (Ab-153) antibody.The picture on the right is treated with the synthesized peptide.