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Anti-CASP9 Antibody E84


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA303420 Rabbit monoclonal antibody against Pro-Caspase-9(E84) 100ul $325 In Stock
LC409796 CASP9 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for CASP9 (NM_032996)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Assay

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near N-terminus of human Caspase-9 was used as immunogen. This antibody only recognises the Pro form of Caspase-9 (46kDa).
Clone NameE84 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:2000; FC: 1:50; IHC-P: 1:25
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified
Note Is unsuitable for ICC or IP.

Reference Data

Target NameHomo sapiens caspase 9, apoptosis-related cysteine peptidase (CASP9), transcript variant 3
Alternative NameAPAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56
Database LinkNP_127463
Entrez Gene 842 Human
FunctionThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq].
Related PathwayProteaseStem cell - PluripotencyDruggable Genome p53 signaling pathwayApoptosisVEGF signaling pathwayAlzheimer's diseaseParkinson's diseaseAmyotrophic lateral sclerosis (ALS)More Pathways >>

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - pro Caspase 9 antibody [E84]; Anti-pro Caspase 9 antibody [E84] at 1/2000 dilution + Jurkat cell lysate at 10 ug.Predicted band size : 46 kDa.Observed band size : 46 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pro Caspase 9 antibody [E84]; Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-9 RabMAb.


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