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Home Antibody All anti-CASP6 antibodies

Anti-CASP6 Antibody EP1325Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300828
  • Rabbit Monoclonal Antibody against CASP6 (Clone EP1325Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400490) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for CASP6 (NM_001226)
cDNA Clone shRNA/siRNA Protein/Lysate Antibody Assay

OriGene Data

ImmunogenA synthetic peptide corresponding to residues of human Caspase-6 was used as immunogen. The antibody should recognize the pro-form of Caspase-6.
Clone NameEP1325Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:20,000; IHC: 1: 100 250; ICC: 1: 100 250; FC: 1:50; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens caspase 6, apoptosis-related cysteine peptidase (CASP6), transcript variant alpha
Alternative NameMCH2
Database LinkNP_001217
Entrez Gene 839 Human
Entrez Gene 12368 Mouse
Entrez Gene 83584 Rat
FunctionCaspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).
Related PathwayProteaseStem cell - PluripotencyDruggable Genome Apoptosis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Caspase 6 antibody [EP1325Y]; Anti-Caspase 6 antibody [EP1325Y] at 1/20000 dilution + MCF-7 cell lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 34 kDa.Observed band size : 34 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Caspase 6 antibody [EP1325Y]; TA300828 at 1/100 dilution staining Caspase 6 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Caspase 6 antibody [EP1325Y]; TA300828 at 1/100 dilution staining Caspase 6 in HeLa cells by Immunofluorescence.
FC Image
Flow Cytometry - Anti-Caspase 6 antibody [EP1325Y]; Overlay histogram showing Hela cells stained with TA300828 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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