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Anti-CASP3 Antibody E83-77
Also for CASP3 (NM_032991)
|A synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit) was used as immunogen. This antibody only detects the active (cleaved) form of Caspase-3 and does not recognize the pro form of Caspase-3.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:500; IHC: 1:250; ICC: 1: 100-250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse, Rat
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Western blot - active Caspase 3 antibody [Y83-77]; Lanes 1 - 2 : anti Pro Caspase 3 at 1/10000 dilution.Lanes 3 - 4 : Anti-active Caspase 3 antibody [E83-77] at 1/500 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Jurkat cell lysate + Camptothecin.Lane 3 : Jurkat cell lysate.Lane 4 : Jurkat cell lysate + Camptothecin.Predicted band size : 32 kDa.Observed band size : 17 kDa .Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - active Caspase 3 antibody [Y83-77]; Ab32042, at dilution of 1/50, staining active Caspase 3 in paraffin embedded human hepatocellular carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - active Caspase 3 antibody [Y83-77]; Ab32042, at dilution of 1/100, staining HeLa cells by Immunofluorescence. .Left image: control. .Right image: staurosporine treated.