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Home Antibody All anti-CASP3 antibodies

Anti-CASP3 Antibody E83-77

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300423
  • Rabbit Monoclonal Antibody against CASP3 (Clone E83-77)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403215) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
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Also for CASP3 (NM_032991)
cDNA Clone shRNA/siRNA Protein/Lysate Antibody Assay

OriGene Data

ImmunogenA synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit) was used as immunogen. This antibody only detects the active (cleaved) form of Caspase-3 and does not recognize the pro form of Caspase-3.
Clone NameE83-77 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500; IHC: 1:250; ICC: 1: 100-250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta
Alternative NameCPP32; CPP32B; SCA-1
Database LinkNP_116786
Entrez Gene 836 Human
FunctionCaspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).
Related Pathway
Apoptosis
MAPK signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - active Caspase 3 antibody [Y83-77]; Lanes 1 - 2 : anti Pro Caspase 3 at 1/10000 dilution.Lanes 3 - 4 : Anti-active Caspase 3 antibody [E83-77] at 1/500 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Jurkat cell lysate + Camptothecin.Lane 3 : Jurkat cell lysate.Lane 4 : Jurkat cell lysate + Camptothecin.Predicted band size : 32 kDa.Observed band size : 17 kDa .Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - active Caspase 3 antibody [Y83-77]; Ab32042, at dilution of 1/50, staining active Caspase 3 in paraffin embedded human hepatocellular carcinoma tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - active Caspase 3 antibody [Y83-77]; Ab32042, at dilution of 1/100, staining HeLa cells by Immunofluorescence. .Left image: control. .Right image: staurosporine treated.

 

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